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验证数据展示

  • IF Staining of Jurkat using 84500-1-RR

    IF Staining of Jurkat using 84500-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SELPLG antibody (84500-1-RR, Clone: 241297G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human tonsillitis using 84500-1-RR

    IHC staining of human tonsillitis using 84500-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84500-1-RR

    IHC staining of human tonsillitis using 84500-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84500-1-RR (SELPLG/CD162 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84500-1-RR

    Affinity and Kinetic Characterization of 84500-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84500-1-RR against Human SELPLG/CD162 were performed. The affinity constant is 0.221 nM.

SELPLG/CD162 Recombinant monoclonal antibody

SELPLG/CD162 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84500-1-RR

产品说明书

CloneNo. 241297G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

SELPLG, 241297G10, CD162, CLA, P-selectin glycoprotein ligand 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84500-1-RR targets SELPLG/CD162 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin P ligand
别名 SELPLG, 241297G10, CD162, CLA, P-selectin glycoprotein ligand 1
计算分子量 412 aa, 43 kDa
GenBank蛋白编号BC029782
基因名称 SELPLG
Gene ID (NCBI) 6404
RRIDAB_3672013
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ14242
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

实验方案

Product Specific Protocols
IF protocol for SELPLG/CD162 antibody 84500-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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