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验证数据展示

  • FC experiment of human peripheral blood leukocyte using PE-FcA98084

    FC experiment of human peripheral blood leukocyte using PE-FcA98084

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of human peripheral blood leukocyte using PE-FcA98084

    FC experiment of human peripheral blood leukocyte using PE-FcA98084

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) (red) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 FcZero-rAbTM Recombinant Antibody for FC
Cat No. PE-FcA98084

产品说明书

CloneNo. 241297F10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-FcA98084 targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 selectin P ligand
别名 SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA
计算分子量 412 aa, 43 kDa
GenBank蛋白编号BC029782
基因名称 SELPLG
Gene ID (NCBI) 6404
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).

实验方案

Product Specific Protocols
FC protocol for PE SELPLG/CD162 antibody PE-FcA98084Download protocol
Standard Protocols
Click here to view our Standard Protocols