验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
28402-1-AP targets SEMA4C in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SEMA4C fusion protein Ag29143 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C |
| 别名 | FLJ20369, KIAA1739, M SEMA F, SEMA4C, SEMACL1, SEMAF, SEMAI, Semaphorin 4C |
| 计算分子量 | 833 aa, 93 kDa |
| 观测分子量 | 93 kDa |
| GenBank蛋白编号 | BC109104 |
| 基因名称 | SEMA4C |
| Gene ID (NCBI) | 54910 |
| RRID | AB_2881134 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9C0C4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA4C antibody 28402-1-AP | Download protocol |
| IHC protocol for SEMA4C antibody 28402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
