SEMA7A Monoclonal antibody
SEMA7A Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3A5G1
验证数据展示
经过测试的应用
| Positive WB detected in | human red blood cells, human placenta tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67397-1-Ig targets SEMA7A in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Immunogen | SEMA7A fusion protein Ag28804 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| Synonyms | CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L |
| Calculated Molecular Weight | 666 aa, 75 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC101643 |
| Gene Symbol | SEMA7A |
| Gene ID (NCBI) | 8482 |
| RRID | AB_2882640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75326 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA7A antibody 67397-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation |
