验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19529-1-AP targets SENP5-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SUMO1/sentrin specific peptidase 5 |
| 别名 | SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, EC:3.4.22.- |
| 计算分子量 | 87 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | NM_152699 |
| 基因名称 | SENP5 |
| Gene ID (NCBI) | 205564 |
| RRID | AB_10643377 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96HI0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| IHC protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| IP protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 | ||
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
iScience Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.
| ||
Front Biosci (Landmark Ed) SUMO-Specific Peptidase 5 Promotes Oesophageal Squamous Cell Carcinoma Growth through the NF-κB-SLC1A3 Axis
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