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Septin 10 Polyclonal antibody

Septin 10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12420-1-AP

Print datasheet

Synonyms

SEPT10, Septin-10, SEPTIN10

验证数据展示

  • IF Staining of HepG2 using 12420-1-AP

    IF Staining of HepG2 using 12420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human kidney using 12420-1-AP

    WB analysis of human kidney using 12420-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12420-1-AP

    IHC staining of human pancreas cancer using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman kidney tissue, HeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12420-1-AP targets Septin 10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Septin 10 fusion protein Ag3071 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 10
Synonyms SEPT10, Septin-10, SEPTIN10
Calculated Molecular Weight 53 kDa, 57 kDa
Observed Molecular Weight 53-57 kDa
GenBank Accession NumberBC020502
Gene Symbol Septin 10
Gene ID (NCBI) 151011
RRIDAB_2186992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0V9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Septin 10 antibody 12420-1-APDownload protocol
IHC protocol for Septin 10 antibody 12420-1-APDownload protocol
IF protocol for Septin 10 antibody 12420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols