验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Pig brain tissue, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68173-1-Ig targets SEPT6 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, mouse, pig, rabbit, rat |
免疫原 | SEPT6 fusion protein Ag32736 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | septin 6 |
别名 | KIAA0128, SEP2, SEPT2, SEPT6, septin 6 |
计算分子量 | 434 aa, 50 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC036240 |
基因名称 | SEPT6 |
Gene ID (NCBI) | 23157 |
RRID | AB_2935265 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q14141 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
实验方案
Product Specific Protocols | |
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WB protocol for SEPT6 antibody 68173-1-Ig | Download protocol |
IF protocol for SEPT6 antibody 68173-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |