验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy SEPT6 Ready-To-Use IHC Kit. SEPT6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68173-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68173 targets SEPT6 in IF/ICC applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, pig, rabbit, rat |
| 免疫原 | SEPT6 fusion protein Ag32736 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | septin 6 |
| 别名 | KIAA0128, SEP2, SEPT2, SEPT6, septin 6 |
| 计算分子量 | 434 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC036240 |
| 基因名称 | SEPT6 |
| Gene ID (NCBI) | 23157 |
| RRID | AB_3084430 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14141 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SEPT6 antibody CL488-68173 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |