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验证数据展示

  • IF Staining of HEK-293 using CL488-68173

    IF Staining of HEK-293 using CL488-68173

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 SEPT6 antibody (CL488-68173, Clone: 1B1G1 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated SEPT6 Monoclonal antibody

SEPT6 Monoclonal Antibody for IF/ICC
Cat No. CL488-68173

Print datasheet

CloneNo. 1B1G1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

IF/ICC

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SEPT6 Ready-To-Use IHC Kit. SEPT6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68173-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68173 targets SEPT6 in IF/ICC applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, pig, rabbit, rat
Immunogen SEPT6 fusion protein Ag32736 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 6
Synonyms KIAA0128, SEP2, SEPT2, SEPT6, septin 6
Calculated Molecular Weight 434 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC036240
Gene Symbol SEPT6
Gene ID (NCBI) 23157
RRIDAB_3084430
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14141
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SEPT6 antibody CL488-68173Download protocol
Standard Protocols
Click here to view our Standard Protocols