• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10769-1-AP

Print datasheet

Synonyms

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein

验证数据展示

  • IF Staining of hTERT-RPE cells using 10769-1-AP

    IF Staining of hTERT-RPE cells using 10769-1-AP

    IF result of anti-Septin 9 (10769-1-AP, 1:200) with hTERT-RPE cell by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Green: SEPT9. Blue: DNA. Fixation in methanol.

  • WB analysis using 10769-1-AP

    WB analysis using 10769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10769-1-AP

    IHC staining of human pancreas cancer using 10769-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • IP experiment of mouse brain using 10769-1-AP

    IP experiment of mouse brain using 10769-1-AP

    IP result of anti-Septin 9 (IP:10769-1-AP, 4ug; Detection:10769-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human colon using 10769-1-AP

    IHC staining of human colon using 10769-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • IF Staining of HeLa using 10769-1-AP

    IF Staining of HeLa using 10769-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 10769-1-AP

    FC experiment of HepG2 using 10769-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Septin 9 (10769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inC6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, drosophila
Immunogen Septin 9 fusion protein Ag1218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 9
Synonyms SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa, 38-40 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
RRIDAB_2185012
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

实验方案

Product Specific Protocols
WB protocol for Septin 9 antibody 10769-1-APDownload protocol
IHC protocol for Septin 9 antibody 10769-1-APDownload protocol
IF protocol for Septin 9 antibody 10769-1-APDownload protocol
IP protocol for Septin 9 antibody 10769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Dev Cell

Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.

Authors - Eva P Karasmanis
  • KD Validated
View Article
canineIF

Curr Biol

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Authors - Eva P Karasmanis
  • KD Validated
View Article
humanWB,IF

Curr Biol

Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.

Authors - Joëlle Salameh
View Article
humanWB, IF

J Cell Biol

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

Authors - Ilona A Kesisova
View Article
humanIF

J Cell Biol

Septins suppress the release of vaccinia virus from infected cells.

Authors - Julia Pfanzelter
View Article
mouseWB

PLoS Genet

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.

Authors - Manoj B Menon
View Article