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验证数据展示

  • IF Staining of HeLa using CL647-10769

    IF Staining of HeLa using CL647-10769

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 Septin 9 antibody (CL647-10769) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-10769

    FC experiment of HepG2 using CL647-10769

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Septin 9 (CL647-10769) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Septin 9 Polyclonal antibody

Septin 9 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-10769

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10769-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10769 targets Septin 9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Septin 9 fusion protein Ag1218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name septin 9
Synonyms SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa, 38-40 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
RRIDAB_3084802
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Septin 9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all of the isoforms about SEPT9.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Septin 9 antibody CL647-10769Download protocol
Standard Protocols
Click here to view our Standard Protocols