验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-10769 targets Septin 9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Septin 9 fusion protein Ag1218 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | septin 9 |
别名 | SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein |
计算分子量 | 64 kDa |
观测分子量 | 65 kDa, 38-40 kDa |
GenBank蛋白编号 | BC021192 |
基因名称 | SEPT9 |
Gene ID (NCBI) | 10801 |
RRID | AB_3084802 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UHD8 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Septin 9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). this antibody can identify all of the isoforms about SEPT9.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 Septin 9 antibody CL647-10769 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |