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SERBP1 Monoclonal antibody, PBS Only

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, Canine

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4H1

Cat No : 67792-1-PBS

Print datasheet

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

验证数据展示

  • WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

    WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.

产品信息

67792-1-PBS targets SERBP1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.

Tested Applications WB, IHC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, pig, Canine
Immunogen SERBP1 fusion protein Ag30202 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SERPINE1 mRNA binding protein 1
Synonyms CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC008045
Gene Symbol SERBP1
Gene ID (NCBI) 26135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NC51
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).