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SERBP1 Monoclonal antibody

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig, Canine

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D4H1

Cat no : 67792-1-Ig

Print datasheet

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1

验证数据展示

  • WB analysis using 67792-1-Ig

    WB analysis using 67792-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 67792-1-Ig

    IHC staining of human liver cancer using 67792-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67792-1-Ig

    IHC staining of human liver cancer using 67792-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67792-1-Ig

    IF Staining of human liver cancer using 67792-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IP experiment of HeLa using 67792-1-Ig

    IP experiment of HeLa using 67792-1-Ig

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

  • IP experiment of HeLa using 67792-1-Ig

    IP experiment of HeLa using 67792-1-Ig

    IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug.

经过测试的应用

Positive WB detected inLNCaP cells, MDCK cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67792-1-Ig targets SERBP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, pig, Canine
Immunogen SERBP1 fusion protein Ag30202 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SERPINE1 mRNA binding protein 1
Synonyms CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC008045
Gene Symbol SERBP1
Gene ID (NCBI) 26135
RRIDAB_2918556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NC51
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

实验方案

Product Specific Protocols
WB protocol for SERBP1 antibody 67792-1-IgDownload protocol
IHC protocol for SERBP1 antibody 67792-1-IgDownload protocol
IF protocol for SERBP1 antibody 67792-1-IgDownload protocol
IP protocol for SERBP1 antibody 67792-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols