验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse heart tissue, MOLT-4 cells, Jurkat cells, K-562 cells, HL-60 cells, rat heart tissue |
Positive IF/ICC detected in | HT-29 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68287-1-Ig targets SERCA3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | SERCA3 fusion protein Ag4527 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ATPase, Ca++ transporting, ubiquitous |
Synonyms | ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3 |
Calculated Molecular Weight | 1043 aa, 114 kDa |
Observed Molecular Weight | 97-110 kDa |
GenBank Accession Number | BC035729 |
Gene Symbol | SERCA3 |
Gene ID (NCBI) | 489 |
RRID | AB_2935368 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q93084 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.
实验方案
Product Specific Protocols | |
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WB protocol for SERCA3 antibody 68287-1-Ig | Download protocol |
IF protocol for SERCA3 antibody 68287-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |