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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

    WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

  • WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

    WB analysis using 68287-1-Ig (same clone as 68287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68287-1-Ig (ATP2A3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 68287-1-Ig (same clone as 68287-1-PBS)

    IF Staining of HT-29 using 68287-1-Ig (same clone as 68287-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SERCA3 antibody (68287-1-Ig, Clone: 1A2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68287-1-PBS in a different storage buffer formulation.

SERCA3 Monoclonal antibody, PBS Only

SERCA3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68287-1-PBS

产品说明书

CloneNo. 1A2C2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68287-1-PBS targets SERCA3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SERCA3 fusion protein Ag4527 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, ubiquitous
别名 ATP2A3, Calcium pump 3, SERCA3, SR Ca(2+) ATPase 3
计算分子量 1043 aa, 114 kDa
观测分子量 97-110 kDa
GenBank蛋白编号BC035729
基因名称 SERCA3
Gene ID (NCBI) 489
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ93084
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The SERCA, which pumps cytosolic Ca2+ into the ER, is an imperative maintainer of ER Ca2+ homeostasis. The mammalian SERCA family is comprised of three tissue-specific members, SERCA1-3. SERCA3 is present in endothelial and epithelial cells of various tissues. SERCA3 helps regulate intracellular Ca2+, which is critical for maintaining normal INS secretion and is closely linked to metabolism, neuronal plasticity, gene transcription, cell growth, differentiation, apoptosis, protein folding and carcinogenesis. Several isoforms of SERCA3 exist due to the alternative splicing.