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SERINC1 Polyclonal antibody

SERINC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20265-1-AP

Print datasheet

Synonyms

KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2

验证数据展示

  • WB analysis of HeLa using 20265-1-AP

    WB analysis of HeLa using 20265-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20265-1-AP (SERINC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20265-1-AP

    IHC staining of human liver cancer using 20265-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 20265-1-AP

    IHC staining of human liver cancer using 20265-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20265-1-AP

    IF Staining of HepG2 using 20265-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20265-1-AP (SERINC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20265-1-AP targets SERINC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine incorporator 1
Synonyms KIAA1253, SERINC1, serine incorporator 1, TDE1L, TDE2, TMS 2
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberNM_020755
Gene Symbol SERINC1
Gene ID (NCBI) 57515
RRIDAB_10693613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERINC1, also named as KIAA1253, TDE1L and TDE2, belongs to the TDE1 family. SERINC1 enhances the incorporation of serine into phosphatidylserine and sphingolipids. The antibody is specific to SERINC1.

实验方案

Product Specific Protocols
WB protocol for SERINC1 antibody 20265-1-APDownload protocol
IHC protocol for SERINC1 antibody 20265-1-APDownload protocol
IF protocol for SERINC1 antibody 20265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols