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验证数据展示

  • IF Staining of HeLa using CL488-67950

    IF Staining of HeLa using CL488-67950

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SERPINB9 antibody (CL488-67950, Clone: 2H9D2 ) at dilution of 1:200.

  • FC experiment of K-562 using CL488-67950

    FC experiment of K-562 using CL488-67950

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SERPINB9 (CL488-67950, Clone:2H9D2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of C6 using CL488-67950

    IF Staining of C6 using CL488-67950

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite® Plus 488 SERPINB9 antibody (CL488-67950, Clone: 2H9D2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SERPINB9 Monoclonal antibody

SERPINB9 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67950

产品说明书

CloneNo. 2H9D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PI9, PI 9, CAP-3, CAP3, CAP 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, C6 cells
Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67950-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67950 targets SERPINB9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SERPINB9 fusion protein Ag6719 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 9
别名 PI9, PI 9, CAP-3, CAP3, CAP 3
计算分子量 42 kDa
观测分子量38 kDa
GenBank蛋白编号BC002538
基因名称 SERPINB9
Gene ID (NCBI) 5272
RRIDAB_2923839
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP50453
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SERPINB9 antibody CL488-67950Download protocol
Standard Protocols
Click here to view our Standard Protocols