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验证数据展示

  • WB analysis using 68319-1-Ig

    WB analysis using 68319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of NCCIT using 68319-1-Ig

    WB analysis of NCCIT using 68319-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 68319-1-Ig

    FC experiment of A549 using 68319-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.4 ug SF4 Monoclonal antibody (68319-1-Ig, Clone:1G9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

SF4 Monoclonal antibody

SF4 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68319-1-Ig

产品说明书

CloneNo. 1G9D8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, NCCIT cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68319-1-Ig targets SF4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SF4 fusion protein Ag8014 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 splicing factor 4
别名 SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858
计算分子量 72 kDa
观测分子量55 kDa, 72 kDa
GenBank蛋白编号BC002986
基因名称 SF4
Gene ID (NCBI) 57794
RRIDAB_3085055
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWZ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF4 antibody 68319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols