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SF4 Monoclonal antibody, PBS Only

SF4 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1G9D8

Cat no : 68319-1-PBS

Print datasheet

Synonyms

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

验证数据展示

  • WB analysis using 68319-1-Ig (same clone as 68319-1-PBS)

    WB analysis using 68319-1-Ig (same clone as 68319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

  • WB analysis of NCCIT using 68319-1-Ig (same clone as 68319-1-PBS)

    WB analysis of NCCIT using 68319-1-Ig (same clone as 68319-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 68319-1-Ig (same clone as 68319-1-PBS)

    FC experiment of A549 using 68319-1-Ig (same clone as 68319-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug SF4 Monoclonal antibody (68319-1-Ig, Clone:1G9D8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

产品信息

68319-1-PBS targets SF4 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen SF4 fusion protein Ag8014 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name splicing factor 4
Synonyms SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC002986
Gene Symbol SF4
Gene ID (NCBI) 57794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWZ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C