SF4 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G9D8

Cat No : 68319-1-PBS

Synonyms

DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4

WB analysis using 68319-1-Ig (same clone as 68319-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.
WB analysis of NCCIT using 68319-1-Ig (same clone as 68319-1-PBS)
NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

Size

68319-1-PBS targets SF4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications	WB, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	SF4 fusion protein Ag8014 种属同源性预测
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Full Name	splicing factor 4
Synonyms	DKFZp434E2216, F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4
Calculated Molecular Weight	72 kDa
Observed Molecular Weight	72 kDa
GenBank Accession Number	BC002986
Gene Symbol	SF4
Gene ID (NCBI)	57794
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8IWZ8
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.