验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, Y79 cells, COLO 320 cells, rat brain tissue, rabbit brain tissue, mouse brain tissue |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66328-1-Ig targets SFRP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, pig, rabbit, mouse |
| 文献引用反应性 | human |
| 免疫原 | SFRP2 fusion protein Ag18840 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | secreted frizzled-related protein 2 |
| 别名 | FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697 |
| 计算分子量 | 295 aa, 34 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC008666 |
| 基因名称 | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2881709 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96HF1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IHC protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IF protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
