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SFRP2 Monoclonal antibody, PBS Only

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3A1

Cat no : 66328-1-PBS

Print datasheet

Synonyms

FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

验证数据展示

  • WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

产品信息

66328-1-PBS targets SFRP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat, pig, rabbit, mouse
Immunogen SFRP2 fusion protein Ag18840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name secreted frizzled-related protein 2
Synonyms FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC008666
Gene Symbol SFRP2
Gene ID (NCBI) 6423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HF1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.