• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SFRP2 Monoclonal antibody, PBS Only

SFRP2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1F3A1

Cat No : 66328-1-PBS

Print datasheet

Synonyms

FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

验证数据展示

  • WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

产品信息

66328-1-PBS targets SFRP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, rat, pig, rabbit, mouse
Immunogen SFRP2 fusion protein Ag18840 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name secreted frizzled-related protein 2
Synonyms FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697
Calculated Molecular Weight 295 aa, 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC008666
Gene Symbol SFRP2
Gene ID (NCBI) 6423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96HF1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.