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SFRS11 Recombinant antibody

SFRS11 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240465B8

Cat no : 83605-6-RR

Print datasheet

Synonyms

SRSF11, Splicing factor, arginine/serine-rich 11, Serine/arginine-rich splicing factor 11, p54, Arginine-rich 54 kDa nuclear protein

验证数据展示

  • FC experiment of HeLa using 83605-6-RR

    FC experiment of HeLa using 83605-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SFRS11 Recombinant antibody (83605-6-RR, Clone:240465B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis using 83605-6-RR

    WB analysis using 83605-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83605-6-RR (SFRS11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83605-6-RR

    IF Staining of HepG2 using 83605-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83605-6-RR

    IF Staining of HepG2 using 83605-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS11 antibody (83605-6-RR, Clone: 240465B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83605-6-RR

    Affinity and Kinetic Characterization of 83605-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83605-6-RR against Human SFRS11 were performed. The affinity constant is 1.37 nM.

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, THP-1 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SRSF11 Ready-To-Use IHC Kit. SRSF11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83605-6-RR targets SFRS11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SFRS11 fusion protein Ag24932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name splicing factor, arginine/serine-rich 11
Synonyms SRSF11, Splicing factor, arginine/serine-rich 11, Serine/arginine-rich splicing factor 11, p54, Arginine-rich 54 kDa nuclear protein
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC040436
Gene Symbol SFRS11
Gene ID (NCBI) 9295
RRIDAB_3671218
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ05519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRS11 (Serine/arginine-rich splicing factor 11) may function in pre-mRNA splicing. It is localized in the cell nucleus and can colocalize with spliceosome components.

实验方案

Product Specific Protocols
WB protocol for SFRS11 antibody 83605-6-RRDownload protocol
IF protocol for SFRS11 antibody 83605-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols