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验证数据展示

  • WB analysis using 60305-1-Ig

    WB analysis using 60305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60305-1-Ig (SGTA antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 60305-1-Ig

    IHC staining of human liver cancer using 60305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with SGTA antibody included
  • IHC staining of human liver cancer using 60305-1-Ig

    IHC staining of human liver cancer using 60305-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60305-1-Ig (SGTA Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SGTA antibody included
  • IF Staining of HeLa using 60305-1-Ig

    IF Staining of HeLa using 60305-1-Ig

    Immunofluorescent analysis of HeLa cells using 60305-1-Ig (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

SGTA Monoclonal antibody

SGTA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60305-1-Ig

产品说明书

CloneNo. 3B10B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, fetal human brain tissue, NIH/3T3 cells, Neuro-2a cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SGTA Ready-To-Use IHC Kit. SGTA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60305-1-Ig targets SGTA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SGTA fusion protein Ag1475 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
别名 Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein
计算分子量 34 kDa
观测分子量 34-37 kDa
GenBank蛋白编号BC008885
基因名称 SGTA
Gene ID (NCBI) 6449
RRIDAB_2881419
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

实验方案

Product Specific Protocols
WB protocol for SGTA antibody 60305-1-IgDownload protocol
IHC protocol for SGTA antibody 60305-1-IgDownload protocol
IF protocol for SGTA antibody 60305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols