验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SGTA Ready-To-Use IHC Kit. SGTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60305-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60305 targets SGTA in IF/ICC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | SGTA fusion protein Ag1475 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha |
| 别名 | Alpha SGT, alphaSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34-37 kDa |
| GenBank蛋白编号 | BC008885 |
| 基因名称 | SGTA |
| Gene ID (NCBI) | 6449 |
| RRID | AB_2883139 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O43765 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SGTA antibody CL488-60305 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |