SH3BGRL Polyclonal antibody
SH3BGRL Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse uterus tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11253-1-AP targets SH3BGRL in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | SH3BGRL fusion protein Ag1780 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SH3 domain binding glutamic acid-rich protein like |
| Synonyms | SH3BGR, SH3 domain-binding glutamic acid-rich-like protein 1, SH3 domain binding glutamic acid rich protein like, Adapter SH3BGRL |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC016709 |
| Gene Symbol | SH3BGRL |
| Gene ID (NCBI) | 6451 |
| RRID | AB_3085369 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75368 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3BGRL, an adaptor protein, is one member of the SH3BGR family. SH3BGRL is ubiquitously expressed in varied human tissues and organs, including the bone marrow, heart, lung, liver, and kidney. SH3BGRL encodes a small protein with a conserved proline-rich PLPPQIF region and two motifs, Homer EVH1-binding and SH3-binding motifs. As a scaffold protein, SH3BGRL could participate in the protein-protein interaction for the integration or/and crosstalk of signal transduction, membrane trafficking, cytoskeletal rearrangements, and other key cellular processes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3BGRL antibody 11253-1-AP | Download protocol |
| IHC protocol for SH3BGRL antibody 11253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
