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  • Featured Product
  • KD/KO Validated

SH3BP1 Polyclonal antibody

SH3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20541-1-AP

Print datasheet

Synonyms

SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1

验证数据展示

  • WB analysis using 20541-1-AP

    WB analysis using 20541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 20541-1-AP

    WB analysis of mouse lung using 20541-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20541-1-AP

    IF Staining of HeLa using 20541-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BP1 antibody (20541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 20541-1-AP

    IHC staining of human stomach cancer using 20541-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20541-1-AP (SH3BP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 20541-1-AP

    IHC staining of human stomach cancer using 20541-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20541-1-AP (SH3BP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, mouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20541-1-AP targets SH3BP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SH3BP1 fusion protein Ag14575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3-domain binding protein 1
Synonyms SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1
Calculated Molecular Weight 701 aa, 76 kDa
Observed Molecular Weight 76-80 kDa, 51 kDa
GenBank Accession NumberBC008282
Gene Symbol SH3BP1
Gene ID (NCBI) 23616
RRIDAB_10694570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3L3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BP1 antibody 20541-1-APDownload protocol
IHC protocol for SH3BP1 antibody 20541-1-APDownload protocol
IF protocol for SH3BP1 antibody 20541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.

Authors - Daniel Schlam
View Article
humanWB

Cancer Med

Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia.

Authors - Kibeom Park
  • KD Validated
View Article
humanWB

J Formos Med Assoc

Prognostic impact of ARHGAP43(SH3BP1) in acute myeloid leukemia

Authors - Li Yang
View Article