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SH3BP1 Monoclonal antibody, PBS Only

SH3BP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12C6

Cat no : 68328-1-PBS

Print datasheet

Synonyms

SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1, 1B12C6

验证数据展示

  • WB analysis using 68328-1-Ig (same clone as 68328-1-PBS)

    WB analysis using 68328-1-Ig (same clone as 68328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    FC experiment of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SH3BP1 Monoclonal antibody (68328-1-Ig, Clone:1B12C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68328-1-Ig (same clone as 68328-1-PBS)

    IF Staining of HeLa using 68328-1-Ig (same clone as 68328-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BP1 antibody (68328-1-Ig, Clone: 1B12C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

产品信息

68328-1-PBS targets SH3BP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SH3BP1 fusion protein Ag15165 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain binding protein 1
Synonyms SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1, 1B12C6
Calculated Molecular Weight 701 aa, 76 kDa
Observed Molecular Weight76-80 kDa
GenBank Accession NumberBC008282
Gene Symbol SH3BP1
Gene ID (NCBI) 23616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3L3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3BP1 is also named as Chimeric SH3BP1-PDXP protein and BARGIN, and belongs to the to the RhoGAP family. SH3BP1 can be as a partner of the exocyst complex and establish a physical and functional link between two motility-driving pathways, the Ral/exocyst and Rac signaling pathways. (PMID:21658605). SH3BP1 localizes together with the exocyst to the leading edge of motile cells and that SH3BP1 regulates cell migration via its GAP activity upon Rac1.