SH3BP5 Monoclonal antibody
SH3BP5 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rabbit, mouse, rat, pig, chicken
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B9D4
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, mouse brain tissue, rabbit brain tissue, chicken brain tissue, Jurkat cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67841-1-Ig targets SH3BP5 in WB, ELISA applications and shows reactivity with Human, rabbit, mouse, rat, pig, chicken samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, rabbit, mouse, rat, pig, chicken |
| Immunogen | SH3BP5 fusion protein Ag30252 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SH3-domain binding protein 5 (BTK-associated) |
| Synonyms | SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC010123 |
| Gene Symbol | SH3BP5 |
| Gene ID (NCBI) | 9467 |
| RRID | AB_2918603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3BP5 antibody 67841-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
