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CoraLite® Plus 488-conjugated SH3BP5 Monoclonal antibody

SH3BP5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit, mouse, rat, pig, chicken

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9D4

Cat no : CL488-67841

Print datasheet

Synonyms

SH3BP-5, SH3BP 5, SH3 domain-binding protein that preferentially associates with BTK, SH3 domain-binding protein 5, SH3 domain binding protein 5

验证数据展示

  • FC experiment of HeLa using CL488-67841

    FC experiment of HeLa using CL488-67841

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SH3BP5 (CL488-67841, Clone:1B9D4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67841-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67841 targets SH3BP5 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat, pig, chicken samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rabbit, mouse, rat, pig, chicken
Immunogen SH3BP5 fusion protein Ag30252 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain binding protein 5 (BTK-associated)
Synonyms SH3BP-5, SH3BP 5, SH3 domain-binding protein that preferentially associates with BTK, SH3 domain-binding protein 5, SH3 domain binding protein 5
Calculated Molecular Weight 47 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC010123
Gene Symbol SH3BP5
Gene ID (NCBI) 9467
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60239
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SH3BP5 antibody CL488-67841Download protocol
Standard Protocols
Click here to view our Standard Protocols