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SH3GLB2 Monoclonal antibody, PBS Only

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B1A10

Cat no : 68325-1-PBS

Print datasheet

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

验证数据展示

  • WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.

产品信息

68325-1-PBS targets SH3GLB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen SH3GLB2 fusion protein Ag8932 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain GRB2-like endophilin B2
Synonyms Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC014635
Gene Symbol SH3GLB2
Gene ID (NCBI) 56904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NR46
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).