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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

SH3GLB2 Monoclonal antibody, PBS Only

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68325-1-PBS

产品说明书

CloneNo. 4B1A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.

产品信息

68325-1-PBS targets SH3GLB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 SH3GLB2 fusion protein Ag8932 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SH3-domain GRB2-like endophilin B2
别名 Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2
计算分子量 395 aa, 44 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC014635
基因名称 SH3GLB2
Gene ID (NCBI) 56904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR46
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).