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验证数据展示

  • WB analysis using 68325-1-Ig

    WB analysis using 68325-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68325-1-Ig

    WB analysis using 68325-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HeLa using 68325-1-Ig

    IP experiment of HeLa using 68325-1-Ig

    IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug.

  • IHC staining of mouse testis using 68325-1-Ig

    IHC staining of mouse testis using 68325-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IHC staining of mouse testis using 68325-1-Ig

    IHC staining of mouse testis using 68325-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SH3GLB2 antibody included
  • IF Staining of HeLa using 68325-1-Ig

    IF Staining of HeLa using 68325-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

SH3GLB2 Monoclonal antibody

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 68325-1-Ig

产品说明书

CloneNo. 4B1A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, rat brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68325-1-Ig targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 SH3GLB2 fusion protein Ag8932 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SH3-domain GRB2-like endophilin B2
别名 Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2
计算分子量 395 aa, 44 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC014635
基因名称 SH3GLB2
Gene ID (NCBI) 56904
RRIDAB_2935397
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NR46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

实验方案

Product Specific Protocols
WB protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IHC protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IF protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IP protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols