CoraLite® Plus 488-conjugated SH3GLB2 Monoclonal antibody
SH3GLB2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4B1A10
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68325-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68325 targets SH3GLB2 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Immunogen | SH3GLB2 fusion protein Ag8932 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SH3-domain GRB2-like endophilin B2 |
| Synonyms | Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2 |
| Calculated Molecular Weight | 395 aa, 44 kDa |
| Observed Molecular Weight | 47-50 kDa |
| GenBank Accession Number | BC014635 |
| Gene Symbol | SH3GLB2 |
| Gene ID (NCBI) | 56904 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NR46 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SH3GLB2 antibody CL488-68325 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |