验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68325 targets SH3GLB2 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human, mouse, rat, pig, rabbit |
免疫原 | SH3GLB2 fusion protein Ag8932 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | SH3-domain GRB2-like endophilin B2 |
别名 | Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2 |
计算分子量 | 395 aa, 44 kDa |
观测分子量 | 47-50 kDa |
GenBank蛋白编号 | BC014635 |
基因名称 | SH3GLB2 |
Gene ID (NCBI) | 56904 |
RRID | AB_3084473 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9NR46 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 SH3GLB2 antibody CL488-68325 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |