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SHARPIN Monoclonal antibody

SHARPIN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A9E2

Cat no : 67177-1-Ig

Print datasheet

Synonyms

DKFZp434N1923, hSIPL1, SHARPIN, SIPL1

验证数据展示

  • WB analysis using 67177-1-Ig

    WB analysis using 67177-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, A549 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67177-1-Ig targets SHARPIN in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SHARPIN fusion protein Ag6367 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SHANK-associated RH domain interactor
Synonyms DKFZp434N1923, hSIPL1, SHARPIN, SIPL1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC025244
Gene Symbol SHARPIN
Gene ID (NCBI) 81858
RRIDAB_2882473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0F6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for SHARPIN antibody 67177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols