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  • Featured Product
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SHC1 Polyclonal antibody

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12496-1-AP

Print datasheet

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

验证数据展示

  • WB analysis using 12496-1-AP

    WB analysis using 12496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12496-1-AP

    WB analysis of HeLa using 12496-1-AP

    WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells.

  • IHC staining of human stomach using 12496-1-AP

    IHC staining of human stomach using 12496-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12496-1-AP

    IF Staining of HeLa using 12496-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 12496-1-AP

    IF Staining of HepG2 using 12496-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12496-1-AP targets SHC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SHC1 fusion protein Ag3165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Synonyms SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa, 52 kDa, 46 kDa
GenBank Accession NumberBC014158
Gene Symbol SHC1
Gene ID (NCBI) 6464
RRIDAB_2187889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29353
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

实验方案

Product Specific Protocols
WB protocol for SHC1 antibody 12496-1-APDownload protocol
IHC protocol for SHC1 antibody 12496-1-APDownload protocol
IF protocol for SHC1 antibody 12496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Exp Cell Res

LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.

Authors - Hao-Ke Li
View Article
humanWB,IF

Sci Adv

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis

Authors - Qiwei Jiang
View Article