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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SHC Polyclonal antibody

SHC Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12496

Print datasheet

Synonyms

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA

验证数据展示

  • IF Staining of HepG2 using CL488-12496

    IF Staining of HepG2 using CL488-12496

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SHC antibody (CL488-12496) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 12496-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12496 targets SHC in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen SHC fusion protein Ag3165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Synonyms SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa, 52 kDa, 46 kDa
GenBank Accession NumberBC014158
Gene Symbol SHC1
Gene ID (NCBI) 6464
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29353
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SHC antibody CL488-12496Download protocol
Standard Protocols
Click here to view our Standard Protocols