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  • Featured Product
  • KD/KO Validated

SHMT1 Polyclonal antibody

SHMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30192-1-AP

Print datasheet

Synonyms

EC:2.1.2.1, CSHMT

验证数据展示

  • WB analysis using 30192-1-AP

    WB analysis using 30192-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30192-1-AP (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 30192-1-AP

    IHC staining of human kidney using 30192-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 30192-1-AP (SHMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHMT1 antibody included
  • IF Staining of HepG2 using 30192-1-AP

    IF Staining of HepG2 using 30192-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT1 antibody (30192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 30192-1-AP

    FC experiment of HeLa using 30192-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHMT1 (30192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, MOLT-4 cells, T-47D cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SHMT1 Ready-To-Use IHC Kit. SHMT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30192-1-AP targets SHMT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SHMT1 fusion protein Ag31269 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine hydroxymethyltransferase 1 (soluble)
Synonyms EC:2.1.2.1, CSHMT
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight45-53 kDa
GenBank Accession NumberBC038598
Gene Symbol SHMT1
Gene ID (NCBI) 6470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

实验方案

Product Specific Protocols
WB protocol for SHMT1 antibody 30192-1-APDownload protocol
IHC protocol for SHMT1 antibody 30192-1-APDownload protocol
IF protocol for SHMT1 antibody 30192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma

Authors - Tianyu Han
  • KD Validated
View Article