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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SHMT1 Polyclonal antibody

SHMT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30192

Print datasheet

Synonyms

EC:2.1.2.1, CSHMT

验证数据展示

  • IF Staining of HepG2 using CL488-30192

    IF Staining of HepG2 using CL488-30192

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SHMT1 antibody (CL488-30192) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-30192

    FC experiment of HeLa using CL488-30192

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SHMT1 Polyclonal antibody (CL488-30192)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SHMT1 Ready-To-Use IHC Kit. SHMT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30192-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30192 targets SHMT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SHMT1 fusion protein Ag31269 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine hydroxymethyltransferase 1 (soluble)
Synonyms EC:2.1.2.1, CSHMT
Calculated Molecular Weight 483 aa, 53 kDa
Observed Molecular Weight45-53 kDa
GenBank Accession NumberBC038598
Gene Symbol SHMT1
Gene ID (NCBI) 6470
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP34896
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SHMT1 antibody CL488-30192Download protocol
FC protocol for CL Plus 488 SHMT1 antibody CL488-30192Download protocol
Standard Protocols
Click here to view our Standard Protocols