SHMT2 Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat No : 67980-1-PBS

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

WB analysis using 67980-1-Ig (same clone as 67980-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67980-1-Ig (same clone as 67980-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

Size

67980-1-PBS targets SHMT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications	WB,Indirect ELISA,IHC Application Description
Tested Reactivity	Human, rat
Immunogen	SHMT2 fusion protein Ag1584 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	serine hydroxymethyltransferase 2 (mitochondrial)
Synonyms	GLYA, Serine methylase, SHMT, SHMT2
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	52-55 kDa
GenBank Accession Number	BC011911
Gene Symbol	SHMT2
Gene ID (NCBI)	6472
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P34897
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.