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验证数据展示

  • WB analysis using 67980-1-Ig

    WB analysis using 67980-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human colon cancer using 67980-1-Ig

    IHC staining of human colon cancer using 67980-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHMT2 antibody included

SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67980-1-Ig

产品说明书

CloneNo. 3A2H9

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, ELISA

别名

GLYA, Serine methylase, SHMT, SHMT2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SHMT2 Ready-To-Use IHC Kit. SHMT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67980-1-Ig targets SHMT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat
免疫原 SHMT2 fusion protein Ag1584 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine hydroxymethyltransferase 2 (mitochondrial)
别名 GLYA, Serine methylase, SHMT, SHMT2
计算分子量 55 kDa
观测分子量52-55 kDa
GenBank蛋白编号BC011911
基因名称 SHMT2
Gene ID (NCBI) 6472
RRIDAB_2918729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP34897
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHMT2 antibody 67980-1-IgDownload protocol
IHC protocol for SHMT2 antibody 67980-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols