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SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat no : 67980-1-Ig

Print datasheet

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

验证数据展示

  • WB analysis using 67980-1-Ig

    WB analysis using 67980-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human colon cancer using 67980-1-Ig

    IHC staining of human colon cancer using 67980-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHMT2 antibody included

经过测试的应用

Positive WB detected inNCCIT cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SHMT2 Ready-To-Use IHC Kit. SHMT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67980-1-Ig targets SHMT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen SHMT2 fusion protein Ag1584 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Synonyms GLYA, Serine methylase, SHMT, SHMT2
Calculated Molecular Weight 55 kDa
Observed Molecular Weight52-55 kDa
GenBank Accession NumberBC011911
Gene Symbol SHMT2
Gene ID (NCBI) 6472
RRIDAB_2918729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP34897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHMT2 antibody 67980-1-IgDownload protocol
IHC protocol for SHMT2 antibody 67980-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols