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CoraLite® Plus 488-conjugated SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A2H9

Cat No : CL488-67980

Print datasheet

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

验证数据展示

  • FC experiment of HepG2 using CL488-67980

    FC experiment of HepG2 using CL488-67980

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SHMT2 (CL488-67980, Clone:3A2H9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67980-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67980 targets SHMT2 in FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, rat
Immunogen SHMT2 fusion protein Ag1584 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine hydroxymethyltransferase 2 (mitochondrial)
Synonyms GLYA, Serine methylase, SHMT, SHMT2
Calculated Molecular Weight 55 kDa
Observed Molecular Weight52-55 kDa
GenBank Accession NumberBC011911
Gene Symbol SHMT2
Gene ID (NCBI) 6472
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP34897
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SHMT2 antibody CL488-67980Download protocol
Standard Protocols
Click here to view our Standard Protocols