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验证数据展示

  • WB analysis using 12441-1-AP

    WB analysis using 12441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12441-1-AP

    IHC staining of human prostate cancer using 12441-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens).

SHPK Polyclonal antibody

SHPK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CARKL, sedoheptulokinase, SHK, SHPK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, RAW 264.7 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12441-1-AP targets SHPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SHPK fusion protein Ag3150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sedoheptulokinase
别名 CARKL, sedoheptulokinase, SHK, SHPK
计算分子量 478 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
RRIDAB_2188566
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

实验方案

Product Specific Protocols
WB protocol for SHPK antibody 12441-1-APDownload protocol
IHC protocol for SHPK antibody 12441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols