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SIAH1 Recombinant antibody, PBS Only

SIAH1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240377B4

Cat no : 83389-4-PBS

Print datasheet

Synonyms

Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1

验证数据展示

  • WB analysis using 83389-4-RR (same clone as 83389-4-PBS)

    WB analysis using 83389-4-RR (same clone as 83389-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • WB analysis of mouse kidney using 83389-4-RR (same clone as 83389-4-PBS)

    WB analysis of mouse kidney using 83389-4-RR (same clone as 83389-4-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83389-4-RR (SIAH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 83389-4-RR (same clone as 83389-4-PBS)

    IHC staining of mouse testis using 83389-4-RR (same clone as 83389-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83389-4-RR (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83389-4-RR (same clone as 83389-4-PBS)

    FC experiment of A549 using 83389-4-RR (same clone as 83389-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Siah1 Recombinant Antibody (83389-4-RR, Clone:240377B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83389-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83389-4-RR

    Affinity and Kinetic Characterization of 83389-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83389-4-RR against Human SIAH1 were performed. The affinity constant is 2.39 nM.

产品信息

83389-4-PBS targets SIAH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SIAH1 fusion protein Ag34618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name seven in absentia homolog 1 (Drosophila)
Synonyms Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC035562
Gene Symbol SIAH1
Gene ID (NCBI) 6477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IUQ4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)