SIAH1 Recombinant antibody

SIAH1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240377B4

Cat no : 83389-4-RR

Print datasheet

Synonyms

Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1



经过测试的应用

Positive WB detected inA549 cells, mouse kidney tissue, HepG2 cells, HEK-293T cells, SH-SY5Y cells, Jurkat cells, COLO 320 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83389-4-RR targets SIAH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SIAH1 fusion protein Ag34618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name seven in absentia homolog 1 (Drosophila)
Synonyms Siah-1a, Siah-1, RING-type E3 ubiquitin transferase SIAH1, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH1
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC035562
Gene Symbol SIAH1
Gene ID (NCBI) 6477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IUQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

实验方案

Product Specific Protocols
WB protocol for SIAH1 antibody 83389-4-RRDownload protocol
IHC protocol for SIAH1 antibody 83389-4-RRDownload protocol
FC protocol for SIAH1 antibody 83389-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols