SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14638-1-AP

Print datasheet

Synonyms

KIAA0700



经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14638-1-AP targets SIN3A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SIN3A fusion protein Ag6219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SIN3 homolog A, transcription regulator (yeast)
Synonyms KIAA0700
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC066364
Gene Symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2301741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96ST3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIN3A antibody 14638-1-APDownload protocol
IF protocol for SIN3A antibody 14638-1-APDownload protocol
IP protocol for SIN3A antibody 14638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

Authors - Ti-Chun Chan
humanWB,CoIP

Int J Biol Sci

TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.

Authors - Kai Qu

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
mouseWB,IP

Nat Commun

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages

Authors - Yinliang Zhang
ratWB

Bioact Mater

Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth

Authors - Miaomei Yu