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验证数据展示

  • WB analysis using 67197-1-Ig

    WB analysis using 67197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 67197-1-Ig

    IF Staining of U2OS using 67197-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SIN3A antibody (67197-1-Ig, Clone: 2B12E8 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

SIN3A Monoclonal antibody

SIN3A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67197-1-Ig

Print datasheet

CloneNo. 2B12E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DKFZp434K2235, FLJ90319, KIAA0700, SIN3A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1250-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67197-1-Ig targets SIN3A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SIN3A fusion protein Ag28865 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SIN3 homolog A, transcription regulator (yeast)
Synonyms DKFZp434K2235, FLJ90319, KIAA0700, SIN3A
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC066364
Gene Symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2882490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96ST3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIN3A antibody 67197-1-IgDownload protocol
IF protocol for SIN3A antibody 67197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols