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验证数据展示

  • FC experiment of A431 using CL488-67197

    FC experiment of A431 using CL488-67197

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIN3A (CL488-67197, Clone:2B12E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated SIN3A Monoclonal antibody

SIN3A Monoclonal Antibody for FC (Intra)
Cat No. CL488-67197

产品说明书

CloneNo. 2B12E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

SIN3A, 2B12E8, KIAA0700

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67197-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67197 targets SIN3A in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 SIN3A fusion protein Ag28865 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SIN3 homolog A, transcription regulator (yeast)
别名 SIN3A, 2B12E8, KIAA0700
计算分子量 145 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC066364
基因名称 SIN3A
Gene ID (NCBI) 25942
RRIDAB_3084323
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96ST3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SIN3A antibody CL488-67197Download protocol
Standard Protocols
Click here to view our Standard Protocols