SIRP Alpha Polyclonal antibody
SIRP Alpha Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | C6 cells, NIH/3T3 cells, U-937 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31485-1-AP targets SIRP Alpha in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | signal-regulatory protein alpha |
Synonyms | BIT, CD172A, Inhibitory receptor SHPS 1, Macrophage fusion receptor, MFR, MYD 1, MyD 1 antigen, MYD1, P84, PTPNS1, SHP substrate 1, SHPS 1, SHPS1, SIRP, SIRP ALPHA 1, Sirp alpha 2, Sirp alpha 3, SIRPA, SIRPalpha, SIRPalpha2 |
Calculated Molecular Weight | 55kd |
Observed Molecular Weight | 65-100 kDa |
GenBank Accession Number | NM_080792 |
Gene Symbol | SIRPA |
Gene ID (NCBI) | 140885 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | P78324 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).
实验方案
Product Specific Protocols | |
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WB protocol for SIRP Alpha antibody 31485-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |