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验证数据展示

  • WB analysis using 31485-1-AP

    WB analysis using 31485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 31485-1-AP

    IF Staining of THP-1 using 31485-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (31485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of THP-1 using 31485-1-AP

    FC experiment of THP-1 using 31485-1-AP

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SIRP Alpha/CD172a Polyclonal antibody

SIRP Alpha/CD172a Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 31485-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

SIRP Alpha, SIRPA, MFR, Inhibitory receptor SHPS-1, CD172a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, NIH/3T3 cells, U-937 cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31485-1-AP targets SIRP Alpha/CD172a in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, MFR, Inhibitory receptor SHPS-1, CD172a
计算分子量55 kDa
观测分子量65-100 kDa
GenBank蛋白编号NM_080792
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_3670002
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP78324
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

实验方案

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 31485-1-APDownload protocol
IF protocol for SIRP Alpha/CD172a antibody 31485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols