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SIRP Alpha/CD172a Monoclonal antibody, PBS Only

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8H3

Cat no : 67337-1-PBS

Print datasheet

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

验证数据展示

  • WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

产品信息

67337-1-PBS targets SIRP Alpha/CD172a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen SIRP Alpha/CD172a fusion protein Ag6361 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name signal-regulatory protein alpha
Synonyms SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC033092
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78324
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.