• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SIRP Alpha/CD172a Monoclonal antibody

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8H3

Cat no : 67337-1-Ig

Print datasheet

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

验证数据展示

  • WB analysis using 67337-1-Ig

    WB analysis using 67337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 67337-1-Ig

    IHC staining of rat cerebellum using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inrat cerebellum tissue, human liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen SIRP Alpha/CD172a fusion protein Ag6361 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name signal-regulatory protein alpha
Synonyms SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC033092
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

实验方案

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
IHC protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols