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验证数据展示

  • WB analysis using 67337-1-Ig

    WB analysis using 67337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 67337-1-Ig

    IHC staining of rat cerebellum using 67337-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SIRP Alpha/CD172a Monoclonal antibody

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67337-1-Ig

产品说明书

CloneNo. 2F8H3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

别名

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inrat cerebellum tissue, human liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 SIRP Alpha/CD172a fusion protein Ag6361 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
计算分子量 55 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC033092
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78324
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

实验方案

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
IHC protocol for SIRP Alpha/CD172a antibody 67337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols