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  • Featured Product
  • KD/KO Validated

SIRT1 Recombinant antibody, PBS Only

SIRT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11

Cat no : 82790-4-PBS

Print datasheet

Synonyms

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-

验证数据展示

  • WB analysis using 82790-4-RR (same clone as 82790-4-PBS)

    WB analysis using 82790-4-RR (same clone as 82790-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82790-4-RR (SIRT1 antibody) at dilution of 1:4700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82790-4-RR (same clone as 82790-4-PBS)

    IF Staining of HepG2 using 82790-4-RR (same clone as 82790-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82790-4-RR (same clone as 82790-4-PBS)

    WB analysis of HeLa using 82790-4-RR (same clone as 82790-4-PBS)

    WB result of SIRT1 antibody (82790-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT1 transfected HeLa cells. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 82790-4-RR (same clone as 82790-4-PBS)

    IP experiment of HEK-293 using 82790-4-RR (same clone as 82790-4-PBS)

    IP result of anti-SIRT1 (IP:82790-4-RR, 4ug; Detection:82790-4-RR 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82790-4-RR

    Affinity and Kinetic Characterization of 82790-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82790-4-RR against Human SIRT1 were performed. The affinity constant is 4.90 nM.

Planning an IHC experiment? We recommend our IHCeasy SIRT1 Ready-To-Use IHC Kit. SIRT1 primary antibody included.

产品信息

82790-4-PBS targets SIRT1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SIRT1 fusion protein Ag3808 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Synonyms NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-
Calculated Molecular Weight 747 aa, 82 kDa
Observed Molecular Weight110-130 kDa
GenBank Accession NumberBC012499
Gene Symbol SIRT1
Gene ID (NCBI) 23411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96EB6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttles between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.