- Featured Product
- KD/KO Validated
SIRT1 Recombinant antibody
SIRT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2F11
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells |
Positive IP detected in | HEK-293 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82790-4-RR targets SIRT1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | human |
Immunogen | SIRT1 fusion protein Ag3808 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Synonyms | NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.- |
Calculated Molecular Weight | 747 aa, 82 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | BC012499 |
Gene Symbol | SIRT1 |
Gene ID (NCBI) | 23411 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96EB6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SIRT1 antibody 82790-4-RR | Download protocol |
IF protocol for SIRT1 antibody 82790-4-RR | Download protocol |
IP protocol for SIRT1 antibody 82790-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |